颁贬翱-碍1/骋α15/颁颁搁1

Figure 1. CCL3-induced concentration-dependent stimulation of intracellular calcium mobilization in 颁贬翱-碍1/骋α15/颁颁搁1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CCR1 agonist, CCL3 (Cat. No. Z03137, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CCL3 (mean ± SEM, n = 3). The EC₅₀ of CCL3 on 颁贬翱-碍1/骋α15/颁颁搁1 cells was 0.63 μg/ml.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ?RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CCL3 in 颁贬翱-碍1/骋α15/颁颁搁1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in 颁贬翱-碍1/骋α15/颁颁搁1 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CCL3 on 颁贬翱-碍1/骋α15/颁颁搁1 cells was 0.32 μg/ml.

Figure 3. FACS analysis of cell surface expression of human CCR1 on 颁贬翱-碍1/骋α15/颁颁搁1 cells. The 颁贬翱-碍1/骋α15/颁颁搁1 cells (green) and the negative control CHO-K1/Gα15 cells (red) were probed using Anti-human CCR1 antibody and Alexa FluorTM 488-conjugated goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 1 (CCR1) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.

产物编号	M00522
规格	2 vials 大单询价
数量
价格	询价


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产物介绍

Product Description	Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 1 (CCR1) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS.
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1. CCL3-induced concentration-dependent stimulation of intracellular calcium mobilization in 颁贬翱-碍1/骋α15/颁颁搁1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CCR1 agonist, CCL3 (Cat. No. Z03137, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CCL3 (mean ± SEM, n = 3). The EC₅₀ of CCL3 on 颁贬翱-碍1/骋α15/颁颁搁1 cells was 0.63 μg/ml.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ?RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CCL3 in 颁贬翱-碍1/骋α15/颁颁搁1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in 颁贬翱-碍1/骋α15/颁颁搁1 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CCL3 on 颁贬翱-碍1/骋α15/颁颁搁1 cells was 0.32 μg/ml.

Figure 3. FACS analysis of cell surface expression of human CCR1 on 颁贬翱-碍1/骋α15/颁颁搁1 cells. The 颁贬翱-碍1/骋α15/颁颁搁1 cells (green) and the negative control CHO-K1/Gα15 cells (red) were probed using Anti-human CCR1 antibody and Alexa FluorTM 488-conjugated goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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