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目录产物 » 稳定细胞系 » Human chemokine (C-X-C motif) receptor 5 (CXCR5) Stable Cell Line
颁贬翱-碍1/骋α15/颁齿颁搁5

Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in 颁贬翱-碍1/骋α15/颁齿颁搁5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on 颁贬翱-碍1/骋α15/颁齿颁搁5 cells was 0.11 μM.

颁贬翱-碍1/骋α15/颁齿颁搁5

Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00526
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产物介绍
Product Description Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • 颁贬翱-碍1/骋α15/颁齿颁搁5
    • 颁贬翱-碍1/骋α15/颁齿颁搁5

    Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in 颁贬翱-碍1/骋α15/颁齿颁搁5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on 颁贬翱-碍1/骋α15/颁齿颁搁5 cells was 0.11 μM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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