| Species |
Human |
| Protein Construction |
FLRT1 (Ile21-Pro524)
Accession # Q9NZU1 |
His |
| N-term |
C-term |
|
| Purity |
>?95%?as?determined?by?Bis?Tris?PAGE
>?95%?as?determined?by?HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized FLRT1, His, Human at 0.5μg/ml (100μl/well) on the plate can bind Anti?FLRT1 Antibody, mFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
56.58 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). |
| Synonyms |
SPG68; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
