Figure 2. Comparative His-tag detection by Western blot was performed using THE? His Antibody, mAb, Mouse (A: GenScript, A00186, at 1 ?g/mL concentration) and Mouse Anti-His mAb (B: Competitor B, at 1 ?g/mL concentration).
Both antibodies were used to probe the same cell lysates containing His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
Researchers can also use MonoRab? Anti-Mouse IgG (H&L) (76F10), mAb, Rabbit (GenScript, V90301) as a secondary antibody.
Figure 7. Immunoprecipitates from cell lysates containing His-tagged fusion protein were analyzed by Western blot using THE? His Antibody, mAb, Mouse (GenScript, A00186).
Lane 1. Positive control containing His-tagged fusion protein
Lane 2. Negative control – IP with isotype control antibody (A01007)
Lane 3. Immunoprecipitation with THE? His Tag Antibody, mAb, Mouse (A00186)
Figure 8. His-tag was detected in Multiple Tag Cell Lysate (GenScript, M0100) by Western blot using THE? His Antibody, mAb, Mouse (GenScript, A00186, 1 ?g/mL).
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
Researchers can also use MonoRab? Anti-Mouse IgG (H&L) (76F10), mAb, Rabbit (GenScript, V90301) as a secondary antibody.
His-tagged fusion protein:
Predicted MW: 52 kDa
Observed MW: 52 kDa
Figure 3. Comparative His-tag detection by Dot blot was performed using THE? His Antibody, mAb, Mouse (A: GenScript, A00186, at 1 ?g/mL concentration) and two Mouse Anti-His mAbs (B: Competitor Q#1, at 1 ?g/mL concentration; C: Competitor Q#2, at 1 ?g/mL concentration).
All three antibodies were used to probe the same samples containing His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
Researchers can also use MonoRab? Anti-Mouse IgG (H&L) (76F10), mAb, Rabbit (GenScript, V90301) as a secondary antibody.
Figure 4. Lot-to-lot consistency of antibody performance was analyzed for 4 batches (Batched 1#, 2#, 3# and 4#) of THE? His Antibody, mAb, Mouse (GenScript, A00186, 1 ?g/mL) by Western blot.
The results show that the antibody-generated signal remains consistent from Lot-to-Lot.
Antibodies from all four lots were used to probe samples of the same His-tagged fusion protein.
The signal was developed with IRDye? 800 Conjugated Goat Anti-Mouse IgG.
Figure 5. Detection of His-tag in CHO cells transfected with His-tagged protein (Green), and non-transfected CHO cells (Black) by flow cytometry using THE? His Tag Antibody, mAb, Mouse (GenScript, A00186).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Figure 1. Comparative His-tag detection by Western blot was performed using THE? His Antibody, mAb, Mouse (A: GenScript, A00186, at 0.1 ?g/mL concentration) and Mouse Anti-His mAb (B: Competitor A, at 0.1 ?g/mL concentration).
Both antibodies were used to probe the same sample containing overexpressed His-tagged fusion protein.
Figure 6. Detection of N- or C-terminal His tags in recombinant fusion proteins were analyzed by Western blot using THE? His Antibody, mAb, Mouse (GenScript, A00186, at 1 ?g/mL concentration).
Lane 1: N-terminal His-tagged fusion protein
Lane 2: C-terminal His-tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
Researchers can also use MonoRab? Anti-Mouse IgG (H&L) (76F10), mAb, Rabbit (GenScript, V90301) as a secondary antibody.
The results show that THE? His Antibody, mAb, Mouse (GenScript, A00186) can recognize N-terminal and C-terminal His-tagged proteins.
BLI (Biolayer interferometry) binding affinity measurements of THE? His Tag Antibody, mAb, Mouse to different His-tagged fusion proteins.
The results show that THE? His Tag Antibody, mAb, Mouse (GenScript, A00186) is able to recognise the His tag at different positions in the protein.
THE? His Tag Antibody, mAb, Mouse
| A00186 | |
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| ￥2500 | |
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