| ￥1600 | |
| A02181-40 | |
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| Specificity | This product is specific for double-stranded RNA (dsRNA) with the length of the helix greater than or equal to 40 bp. The dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. It can bind dsRNA (Non-modified), dsRNA (N1-methyl-Pseudouridine, m1Ψ), dsRNA (Pseudouridine, ψ), dsRNA (5-Methyl-Cytidine, m5C) and dsRNA (5-methoxyuridine, 5moU). |
| Host Species | Mouse |
| Immunogen | Double-stranded RNA |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2a, κ |
| Clonality | Monoclonal |
| Clone ID | J2 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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Figure 1. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (Non-modified) (GenScript, M01145)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K1) [Biotin], mAb, Mouse (GenScript, A05710)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.041-10 ng/ml.
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Figure 2. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (Non-modified) (GenScript, M01145)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K2) [Biotin], mAb, Mouse (GenScript, A05709)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.041-10 ng/ml.
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Figure 3. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (Non-modified) (GenScript, M01145)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (J2) [Biotin], mAb, Mouse (GenScript, A02356)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.062-5 ng/ml.
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Figure 4. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (m1Ψ) (GenScript, M01146)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K1) [Biotin], mAb, Mouse (GenScript, A05710)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.069-50 ng/ml.
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Figure 5. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (m1Ψ) (GenScript, M01146)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K2) [Biotin], mAb, Mouse (GenScript, A05709)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.137-100 ng/ml.
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Figure 6. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (m1Ψ) (GenScript, M01146)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (J2) [Biotin], mAb, Mouse (GenScript, A02356)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.041-30 ng/ml.
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Figure 7. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (5moU) (GenScript, M01152)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K1) [Biotin], mAb, Mouse (GenScript, A05710)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.037-30 ng/ml.
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Figure 8. The dsRNA ELISA assay can be developed with below materials
dsRNA Standard: dsRNA Standard-400bp (5moU) (GenScript, M01152)
Capture antibody: Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse (GenScript, A02181)
Detection antibody: Double-stranded RNA (dsRNA) Antibody (K2) [Biotin], mAb, Mouse (GenScript, A05709)
Streptavidin-HRP (GenScript, M00091) is used for signal development.
The test range for dsRNA is between 0.25-20 ng/ml.
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Figure 9. The results showed that J2 antibody (GenScript, A02181), J2 antibody [Biotin] (GenScript, A02356) and J2 antibody [HRP] (GenScript, A02357) can be used for dsRNA Dot Blot assay development.
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| Target Background | Double-stranded RNA (dsRNA) forms in nucleic acid strands containing complementary sequences. In gene therapy, minimization and removal of double-stranded RNA (dsRNA) is important for mRNA in vitro transcription (IVT) processes. Anti-dsRNA monoclonal antibody J2 is a useful tool in dsRNA detection. J2 antibody also can be used to detect dsRNA intermediates of Hepatitis C virus, Dengue virus, rhinovirus and Chikungunya virus. Since it was first published in 1991, J2 antibody has been broadly widely used to detect viral dsRNA in animals and plants in more than 200 scientific publications. The original J2 antibody was prepared by immunization of mice with L-dsRNA. GenScript Double-stranded RNA (dsRNA) Antibody (J2), mAb, Mouse is recombinant mouse monoclonal antibody, purified from cell culture free from animal-derived components. |
| Synonyms | Double-stranded RNA |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
