| ￥2500 | |
| A02361-200 | |
|
|
|
|
|
|
|
|
|
| Specificity |
This VHH is a negative VHH developed from Alpaca VHH library (Immunogen is not proteins of natural origin but is synthesized through genetic engineering techniques, and this VHH control also not binds to the immunogen either). It serves as a VHH isotype control and is compatible with GenScript anti-VHH antibodies (e.g., A01860, A02165, A02014) for sandwich ELISA applications. |
| Host Species | Alpaca |
| Immunogen | Not applicable |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||
|
|||||
| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.2), 0.03% ProClin 300 and 50% glycerol. |
| Concentration | 0.5 mg/ml |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
His-tag affinity purification.
Note: The product contains a His tag for purification purposes. |
| Isotype | VHH |
| Clonality | Monoclonal |
| Clone ID | Not applicable |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
The purity of Alpaca VHH Control, His Tag (GenScript, A02361) is greater than 95% as determined by SEC-HPLC. ?
Alpaca VHH Control, His Tag (GenScript, A02361) on SDS-PAGE under reducing and non-reducing conditions. The purity is greater than 95%. ?
Flow cytometry results show that Alpaca VHH Control, His Tag (GenScript, A02361) exhibits minimal non-specificity for HEK-293, Raji and Jurkat cells at a concentration of 10 μg/mL. THE? His Tag Antibody [PE], mAb, Mouse (GenScript, A02247) was utilized as the detection antibody at a concentration of 10 μg/mL. ?
Standard curve of Alpaca VHH Control, His Tag (GenScript, A02361) detected by sandwich ELISA.
MonoRab? Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) was coated at a concentration of 1 μg/mL, followed by the addition of Alpaca VHH Control, His Tag at serially diluted concentrations starting from 1 μg/mL. Detection was performed using MonoRab? Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) diluted at 1:5,000. This assay exhibits a sensitivity of up to 0.46 ng/mL for A02361. ?
| Target Background | This product is an isotype control and does not recognize any specific target antigen. It is used as a negative control to assess non-specific binding and background signals in immunoassays. |
| Synonyms | Not applicable |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
