Llama VHH Control, His Tag
Llama VHH Control, His Tag specifically binds to HIV P24 Protein. It serves as a VHH isotype control and is compatible with GenScript anti-VHH antibodies for sandwich ELISA applications.
| ￥2500 | |
| A05708-200 | |
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| ￥2500 | |
| A05708-200 | |
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| Specificity |
This product specifically binds to HIV P24 Protein. It serves as a VHH isotype control and is compatible with GenScript anti-VHH antibodies (e.g., A01860, A02165, A02014) for sandwich ELISA applications. |
| Host Species | Llama |
| Immunogen | HIV p24 Protein |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.2), 0.03% ProClin 300 and 50% glycerol. |
| Concentration | 0.5 mg/ml |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
His-tag affinity purification.
Note: The product contains a His tag for purification purposes. |
| Isotype | VHH |
| Clonality | Monoclonal |
| Clone ID | 59H10 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
Llama VHH Control, His Tag (GenScript, A05708) on SDS-PAGE under reducing conditions. The purity is greater than 95%. ?
BLI (Biolayer interferometry) binding affinity measurements of Llama VHH Control, His Tag (GenScript, A05708) to HIV p24 Protein, His Tag (GenScript, Z06797).
The Llama VHH Control was captured onto biosensor probes immobilized with MonoRab? Rabbit Anti-Camelid VHH Cocktail (Cat. No. A02014) and demonstrated binding to HIV p24 protein with a dissociation constant (KD) of 2.92E-010M. ?
The binding curve between Llama VHH Control, His Tag (GenScript, A05708) and HIV p24 Protein, His Tag (GenScript, Z06797).
HIV p24 Protein, His Tag was coated at a concentration of 1 μg/mL, followed by the addition of Llama VHH Control, His Tag at serially diluted concentrations starting from 1 μg/mL. Detection was performed using MonoRab? Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) diluted at 1:5,000. ?
| Target Background | HIV-1 p24 is a core capsid protein essential for viral assembly and early-stage replication. Its stable structure helps shield viral RNA from host immune detection, contributing to immune evasion. As a highly conserved and abundant biomarker, p24 is widely used in early HIV diagnostics. In the field of cell and gene therapy (CGT), p24 also serves as a key indicator for lentiviral vector quantification, offering a reliable measure of viral titer during vector production and quality control. |
| Synonyms | HIV-1 capsid protein p24; HIV-1 p24 antigen; HIV-1 CA protein; HIV-1 core protein p24; HIV-1 Gag p24 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
