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目录产物 » GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse
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GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse

This product is specific for Human CD3
询价
GMPA02199-100

产物概述
Specificity This product is specific for Human CD3
Host Species Mouse
Immunogen Recombinant Human CD3
Conjugate Unconjugated
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应用指导
ex vivo cell culture process; Flow cytometry
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产物属性
Form Liquid
Storage Buffer Supplied in 10 mM Sodium Citrate, 150 mM NaCl, 0.02 mg/ml Polysorbate 80, pH 5.0
Concentration 5 mg/ml
Storage Instructions The product remains stable at -70 ± 10 °C. Avoid repeated freeze-thaw cycles. Please refer to the COA for specific expiry date.
Isotype Mouse IgG2a
Clonality Monoclonal
Clone ID OKT3
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图例
GMP Anti-Human CD3 Antibody (OKT3), MAb, Mouse

GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) was used for primary T cell activation together with anti-Human CD28 antibody. The percentage of activated T cells was measured by flow cytometry. Data showed that GenScript GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse outperformed the competing products in activating primary T cells. ?

GMP Anti-Human CD3 Antibody (OKT3), MAb, Mouse

Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) and GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) for 6 h. Receptor-mediated signaling induced luminescence via the activation of the IL-2 pathway. ?

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质控参数
外观 Clear, colorless liquid
pH 5.0 ± 0.5
Concentration 5.0 ± 0.5 mg/ml tested by A280 nm
Purity ≥ 95.0% as analyzed by SEC-HPLC
Sequence The coverage of the light chain and heavy chain sequence was 100% consistent with the theoretical amino acid sequence by mass spectrometry (MS)
Residual HCP ≤ 100.0 ng/mg by ELISA method
Residual HCD ≤ 10.0 pg/mg by quantitative PCR method
Residual Protein A ≤ 10.0 ng/mg by ELISA method
Endotoxin Level < 0.1 EU/mg by gel clotting method
Sterility No growth
Reactivity Qualified
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技术背景
Target Background CD3 is a member of the immunoglobulin superfamily, expressed on T cells. It is composed of four distinct chains (ε, γ, δ, ζ). CD3 antigen can form a complex with the T cell receptor (TCR) to generate an activation signal in T cells, which plays an important role in the transduction of TCR signal and the activation of both cytotoxic T cells (CD8 + na?ve T cells) and T helper cells (CD4+ na?ve T cells).
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For laboratory research and ex vivo cell isolation and culture applications. Direct human use, including taking orally and injection and clinical use are forbidden.