| ￥2800 | |
| RC00004-100 | |
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| Description | PE2 is a revolutionary genome editing technology that allows for precise genetic modifications with minimal unintended effects. It integrates a Cas9-nickase fusion protein with a reverse transcriptase and a prime-editing guide RNA (pegRNA), enabling flexible editing capabilities.PE 2 represents a cutting-edge development in genome editing technology, offering significant advantages over traditional methods in terms of accuracy and versatility.? |
| single guide RNA (sgRNA) dependent single-stranded DNA cleavage |
| References | David R Liu,et al. “Search-and-replace genome editing without double-strand breaks or donor DNA”. Nature. 2019 Dec;576(7785):149-157. |
| Purity | ≥90% by SDS-PAGE, 83% by SEC-HPLC |
| Residual DNase | Non-specific DNase activity |
| Endotoxin Level | Control <10EU/mg |
| Expression System | Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | N-His tag |
| Molecular Weight | 242.057KDa |
| Storage & Stability | PE2 is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C. |
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