ABE8e

目录产物 » Adenine base editor 8e

ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.? ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing.

产物编号	RC00010
规格	30 ?g (3mg/ml) 100 μg(10mg/ml) 500 μg(10mg/ml) 5 mg(10mg/ml) 大单询价
数量
价格	￥1400


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产物概述

Description

应用指导

single guide RNA (sgRNA) dependent single-stranded DNA cleavage

技术背景

References

David R Liu,et al.” Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity” Nat Biotechnol. 2020 Jul;38(7):883-891.

质控参数

Purity	≥90% by SDS-PAGE, 49% by SEC-HPLC
Residual DNase	Non-specific DNase activity
Endotoxin Level	Control <10EU/mg

产物属性

Expression System	Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli
Species	S. pyogenes
Tag	N-His tag
Molecular Weight	185.140 Kda
Storage & Stability	ABE8e is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.