High Affinity Ni-NTA Resin

鐩綍浜у搧 » High Affinity Ni-NTA Resin

灞傛瀽鏌卞畨瑁呮祦绋嬬ず鎰忓浘

狈颈-狈罢础浜插拰灞傛瀽浠嬭川锛堜骇鍝佺紪鍙稬00250锛夋槸鎶奛罢础锛堟爱宸濅笁涔欓吀锛夊叡浠峰伓鑱斿埌4%鐞艰刚绯栦粙璐ㄤ笂锛屽啀閫氳绷狈罢础鐨�4涓粨鍚堜綅镣硅灟鍚圢颈2+鍒跺钥屾垚鐨勪翰鍜屽眰鏋愪粙璐ㄣ赌侼颈-狈罢础浜插拰灞傛瀽浠嬭川鐨凬颈2+鑴辫惤鐜囱缉浣庯紝瀹冭兘钥愬弹铔嬬櫧绾寲杩囩▼涓娇鐢ㄧ殑寰堝娣诲姞鍓傦紝骞朵笖链夌潃杈冮珮鐨勮泲鐧界粨鍚堣兘鍔涘拰绋冲畾镐э紝鍥犳狈颈-狈罢础浜插拰灞傛瀽浠嬭川闈炲父閫傜敤浜庡缁勬皑閰搁吨缁勮泲鐧界殑绾寲銆�

浜у搧缂栧彿	L00250
瑙勬牸	10 ml Resin (Total 20 ml) 10 ml Resin (with 5 columns) 25 ml Resin (Total 50 ml) 50 ml Resin (Total 100 ml) 100 ml Resin (Total 200 ml) 200 ml Resin (Total 400 ml) 澶у崟璇环
鏁伴噺
浠锋牸	锟�570


鑱旂郴鎴戜滑

Description

狈颈-狈罢础浜插拰灞傛瀽浠嬭川锛堜骇鍝佺紪鍙稬00250锛夋槸鎶奛罢础锛堟爱宸濅笁涔欓吀锛夊叡浠峰伓鑱斿埌4%鐞艰刚绯栦粙璐ㄤ笂锛屽啀閫氳绷狈罢础鐨�4涓粨鍚堜綅镣硅灟鍚圢颈²⁺鍒跺钥屾垚鐨勪翰鍜屽眰鏋愪粙璐ㄣ赌侼颈-狈罢础浜插拰灞傛瀽浠嬭川鐨凬颈²⁺鑴辫惤鐜囱缉浣庯紝瀹冭兘钥愬弹铔嬬櫧绾寲杩囩▼涓娇鐢ㄧ殑寰堝娣诲姞鍓傦紝骞朵笖链夌潃杈冮珮鐨勮泲鐧界粨鍚堣兘鍔涘拰绋冲畾镐э紝鍥犳狈颈-狈罢础浜插拰灞傛瀽浠嬭川闈炲父閫傜敤浜庡缁勬皑閰搁吨缁勮泲鐧界殑绾寲銆�

浜у搧姒傝堪

Key Features

浜у搧鐗瑰緛

鍩鸿川	4%鐞艰刚绯�
骞冲潎绮掑缎	90 渭m锛�45-165 渭m锛�
鍚搁檮閲�	鈮�20 mg 6xHis-tagged 铔嬬櫧锛�27 kDa锛�
鍌ㄥ瓨婧舵恫	鍚�20%涔欓唶鐨�1脳笔叠厂

狈颈-狈罢础浜插拰灞傛瀽浠嬭川鍙赌愬弹璇曞墏

鍙樻赌у墏	琛ㄩ溃娲绘赌у墏	杩樺师鍓�	鐩�	鍏朵粬&苍产蝉辫;
6 M 鐩愰吀鑳�	2%Triton X-100	20 mM 尾-ME	4 M MgCl₂	50%鐢樻补
8 M灏跨礌	2% Tween 20	1 mM DTT	5 mM CaCl₂	20%涔欓唶
鈥斺赌�	1% CHAPS	鈥斺赌�	2 M NaCl	1 mM EDTA

浜у搧灞炴赌�

Storage & Stability

2-8鈩冿紱DO NOT FREEZE

搴旂敤鎸囧

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

鍥句緥

灞傛瀽鏌卞畨瑁呮祦绋嬬ず鎰忓浘