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Intended Use
AmMag? Ultra AT protein A magbeads are developed and optimized for antibody purification for high throughput and high volume applications.

Principle
The sample containing antibody is added to the AmMag? Ultra AT protein A magbeads and incubated on a rotor overnight or 2 hours (depending on the concentration of samples) for the antibody to bind to the beads. The antibody bound to the beads can be eluted off by using acidic elution facilitated by a magnetic separation device. Magnetic separation eliminates the need for centrifugation, minimizes the loss of sample and makes the process more user friendly.

Material Supplied
GenScript AmMag? Ultra AT protein A magbeads are super paramagnetic beads with diameter of 45-100 μm, covalently coated with Ultra alkaline tolerant protein A, After treatment with 1M NaOH for 24h, the load decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol. The AmMag? Ultra AT protein A magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in binding load.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s, mainly through the Fc regions. The alkaline tolerant protein A not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also tolerates alkaline conditions and can withstand rigorous cleaning procedures.