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CPF1/Cas12a mRNA (Cap1, m1Ψ)

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. LbCpf1 increases homology-directed genome editing. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudouridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
￥1000
RP-A00004-0.1

Description

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communication, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
产物属性
Form Liquid
Concentration 1mg/mL
Full mRNA length 4047nt
Full mRNA Molecular Weight 1313929
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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质控参数
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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应用指导
Transfect a total of 0.5-1.5??g of CPF1/Cas12a mRNA and crRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10? cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.