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TIM-3 Fc Chimera, Human

TIM-3 Fc Chimera, Human

TIM-3 Fc Chimera, Human

Immobilized Galectin-9 His,Human at 0.5μg/mL (100 μL/well) can bind TIM-3 Fc,Human with a linear range of 0.78-6.25 μg/mL.

TIM-3 Fc Chimera, Human

Measured by its binding ability in a functional ELISA. Immobilized recombinant human TIM-3 at 500 ng/mL, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5 ng/mL.

TIM-3 Fc Chimera, Human

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens.
Z03392
￥2200

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产物介绍
Species Human
Protein Construction
TIM-3 (Ser22-Arg200)
Accession # Q8TDQ0		 hFc
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Assay #1: Immobilized TIM-3, hFc, Human at 0.5 μg/ml, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/ml.
Assay #2: Immobilized Galectin-9, His, Human at 0.5 μg/ml (100 ?l/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/ml.
Expression System HEK 293
Apparent Molecular Weight 60~65 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例
  • TIM-3 Fc Chimera, Human
    • TIM-3 Fc Chimera, Human

  • TIM-3 Fc Chimera, Human
    • TIM-3 Fc Chimera, Human

  • TIM-3 Fc Chimera, Human
    • TIM-3 Fc Chimera, Human

    Immobilized Galectin-9 His,Human at 0.5μg/mL (100 μL/well) can bind TIM-3 Fc,Human with a linear range of 0.78-6.25 μg/mL.

  • TIM-3 Fc Chimera, Human
    • TIM-3 Fc Chimera, Human

    Measured by its binding ability in a functional ELISA. Immobilized recombinant human TIM-3 at 500 ng/mL, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5 ng/mL.


技术背景
Target Background T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens.
Synonyms Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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