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目录产物 » GenCRISPR™/Cas9 基因编辑相关产物 » 颁补蝉9核酸酶 » GenCrispr T7 Endonuclease I
GenCrispr T7 Endonuclease I

The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.

GenCrispr T7 Endonuclease I

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5? to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.
Z03396
￥550

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Description

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5? to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.

Synonyms

T7E1

Note

T7 Endonuclease I is a structure-selective enzyme. It acts on a variety of DNA substrates with different specific activities. It is important to control the amount of enzyme and the reaction time used for cleavage of a particular substrate. Temperatures above 42°C cause an increase in nonspecific nuclease activity and should be avoided.

应用指导
  • Resolve four-way junction or branched DNA.
  • Detect or cleave hetero duplex and nicked DNA.
  • Randomly cleave linear DNA for shot-gun cloning.
  • Detect gene mutagenesis and SNPs, for cleavage efficiency assays induced by TALEN,CRISPR/CAS9 or other gene editing tools.

产物属性
Storage & Stability GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl（pH 7.5）, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C.

图例
  • GenCrispr T7 Endonuclease I
    • GenCrispr T7 Endonuclease I

    The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.


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