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目录产物 » CD73/NT5E hFc Chimera, Human
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CD73/NT5E hFc Chimera, Human

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
￥3500
Z04222-100

产物介绍
Species Human
Protein Construction
CD73/NT5E (Trp27-Lys547)
Accession # P21589-1		 hFC
N-term C-term
Purity >?95%?as?determined?by?Bis?Tris?PAGE?
>?95%?as?determined?by?HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD73/NT5E hFc Chimera, Human at 2μg/ml (100μl/well) on the plate can bind Biotinylated Anti?CD73 Antibody, hFc Tag. Test result was comparable to standard batch. Measured by its ability to hydrolyze the 5'?phosphate group from the substrate adenosine?5'?monophosphate (AMP). The specific activity is >10000 pmol/min/μg. Test result meets the standard
Expression System HEK293
Theoretical Molecular Weight 84.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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技术背景
Target Background CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Synonyms CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.