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目录产物 » CD73/NT5E, His, Mouse
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CD73/NT5E, His, Mouse

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside?5'?monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
￥3000
Z04327-100

产物介绍
Species Mouse
Protein Construction
CD73/NT5E (Trp29-Lys549)
Accession # Q61503		 His
N-term C-term
Purity >?95%?as?determined?by?Bis?Tris?PAGE
>?95%?as?determined?by?HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD73/NT5E, His, Mouse at 2μg/ml (100μl/well) on the plate can bind Anti?CD73 Antibody, hFc Tag. Test result was comparable to standard batch. Measured by its ability to hydrolyze the 5'?phosphate group from the substrate adenosine?5'?monophosphate (AMP). The specific activity is >10000 pmol/min/μg. Test result meets the standard.
Expression System HEK293
Theoretical Molecular Weight 58.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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技术背景
Target Background CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside?5'?monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Synonyms CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.