| ￥5800 | |
| Z04409-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
>?95%?as?determined?by?Bis?Tris?PAGE? >?95%?as?determined?by?HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized B7-H4[Biotin], His & Avi, Human at 2μg/ml (100μl/Well) on streptavidin(5μg/ml) precoated plate can bind Anti?B7?H4 Antibody, hFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 28.2 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized B7-H4[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7 ng/ml determined by ELISA. ?
The purity of B7-H4[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. ?
B7-H4[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
| Target Background | B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status. |
| Synonyms | B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
