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目录产物 » CTLA-4/CD152[Biotin], His & Avi, Human
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CTLA-4/CD152[Biotin], His & Avi, Human

CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an 'off' switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
￥3300
Z04436-100

产物介绍
Species Human
Protein Construction
CTLA-4/CD152 (Lys36-Asp161)
Accession # P16410		 His Avi
N-term C-term
Conjugate Biotin
Purity >?95%?as?determined?by?Bis?Tris?PAGE?
>?95%?as?determined?by?HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CTLA-4/CD152[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human B7?1, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CTLA-4/CD152[Biotin], His & Avi, Human

Immobilized CTLA-4/CD152[Biotin], His & Avi, Human His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA. ?

CTLA-4/CD152[Biotin], His & Avi, Human

Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for CTLA-4/CD152[Biotin], His & Avi, Human, His Tag with the EC50 of 45.7 ng/ml determined by ELISA. ?

CTLA-4/CD152[Biotin], His & Avi, Human

The purity of CTLA-4/CD152[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. ?

CTLA-4/CD152[Biotin], His & Avi, Human

CTLA-4/CD152[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?

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技术背景
Target Background CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an 'off' switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.