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目录产物 » GPA33/A33[Biotin], His & Avi, Human
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GPA33/A33[Biotin], His & Avi, Human

Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
￥5800
Z04476-100

产物介绍
Species Human
Protein Construction
GPA33/A33 (Ile22-Val235)
Accession # Q99795-1		 His Avi
N-term C-term
Conjugate Biotin
Purity >?95%?as?determined?by?Bis?Tris?PAGE?
>?95%?as?determined?by?HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized GPA33/A33[Biotin], His & Avi, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti?GP33 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
GPA33/A33[Biotin], His & Avi, Human

Immobilized GPA33/A33[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA. ?

GPA33/A33[Biotin], His & Avi, Human

The purity of GPA33/A33[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. ?

GPA33/A33[Biotin], His & Avi, Human

GPA33/A33[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?

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技术背景
Target Background Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
Synonyms Glycoprotein A33; GPA33; A33; MGC129986; MGC129987
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.