| ￥3500 | |
| Z04803-100 | |
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| Species | Cynomolgus | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized GITR/TNFRSF18, His, Cynomolgus at 0.5μg/ml (100μl/well) on the plate can bind Anti?GITR Antibody, hFc Tag. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 15.87 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized GITR/TNFRSF18, His, Cynomolgus, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA. ?
GITR/TNFRSF18, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind GITR/TNFRSF18, His, Cynomolgus, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200). ?
| Target Background | GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. |
| Synonyms | CD357; GITR; GITR-D; TNFRSF18; AITR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
