| ￥3300 | |
| Z04919-100 | |
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| Species | Cynomolgus | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized SOST/Sclerostin, His, Cynomolgus at 1μg/ml (100μl/well) on the plate can bind Human LRP?6, mFc Tag. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 22.58 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized SOST/Sclerostin, His, Cynomolgus, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 11.6 ng/ml determined by ELISA. ?
SOST/Sclerostin, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
Immobilized SOST/Sclerostin, His, Cynomolgus, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. ?
| Target Background | SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
| Synonyms | sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
