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DKK1, His, Human

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
￥1800
Z05219-100

产物介绍
Species Human
Protein Construction
DKK1 (Thr32-His266)
Accession # O94907		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized DKK1, His, Human at 5μg/ml (100μl/well) on the plate can bind Human LRP?5, mFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.87 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
DKK1, His, Human

Immobilized DKK1, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.50 μg/ml determined by ELISA. ?

DKK1, His, Human

DKK1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?

DKK1, His, Human

Immobilized DKK1, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.42 μg/ml determined by ELISA. ?

DKK1, His, Human

Immobilized DKK1, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. ?

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技术背景
Target Background Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Synonyms Dickkopf-1; Dkk1; SK
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.