| ￥3000 | |
| Z05388-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human BTLA, His Tag at 1μg/ml (100 μl/Well) on the plate can bind HVEM/TNFRSF14 hFc Chimera, Human. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 44.3 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human BTLA, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for HVEM/TNFRSF14 hFc Chimera, Human, hFc Tag with the EC50 of 27.2 ng/ml determined by ELISA. ?
HVEM/TNFRSF14 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
Immobilized Human LIGHT, His Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for HVEM/TNFRSF14 hFc Chimera, Human, hFc Tag with the EC50 of 18.2 ng/ml determined by ELISA. ?
Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 μM as determined in SPR assay (Biacore T200). ?
| Target Background | Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. |
| Synonyms | CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
