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目录产物 » IL-13Ra1 hFc Chimera, Human
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IL-13Ra1 hFc Chimera, Human

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
￥1800
Z05437-100

产物介绍
Species Human
Protein Construction
IL-13Ra1 (Ala27-Thr343)
Accession # P78552		 hFc
N-term C-term
Purity >?95%?as?determined?by?Bis?Tris?PAGE?
> 95%?as?determined?by?HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity IL-13Ra1 hFc Chimera, Human captured on CM5 Chip via Protein A can bind Human IL?13, His Tag in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 63.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
IL-13Ra1 HFc Chimera, Human

The purity of IL-13Ra1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. ?

IL-13Ra1 HFc Chimera, Human

IL-13Ra1 hFc Chimera, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. ?

IL-13Ra1 HFc Chimera, Human

IL-13Ra1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 9.47 nM as determined in SPR assay (Biacore T200). ?

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技术背景
Target Background Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Synonyms IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.