Species | Human | ||||
Protein Construction |
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Purity |
>?95%?as?determined?by?Bis?Tris?PAGE? > 95%?as?determined?by?HPLC |
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Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
Biological Activity | Human B7?H5, hFc Tag captured on CM5 Chip via Protein A can bind VSIG3/IGSF11, His, Human in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||
Expression System | HEK293 | ||||
Theoretical Molecular Weight | 24.31 kDa | ||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. | ||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of VSIG3/IGSF11, His, Human is greater than 95% as determined by SEC-HPLC. ?
VSIG3/IGSF11, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
VSIG3/IGSF11, His, Human on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 μM as determined in SPR assay (Biacore T200). ?
Target Background | Immunoglobulin superfamily member 11 (IgSF11), a homophilic adhesion molecule that preferentially expressed in the brain, is a dual-binding partner of the postsynaptic scaffolding protein PSD-95 and AMPA glutamate receptors (AMPARs). |
Synonyms | IgSF11; Bt-IGSF; Bt IGSF; BtIGSF; VSIG3; CXADRL1; CT119 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.