| ￥3500 | |
| Z06119-100 | |
|
|
|
|
|
|
|
|
|
| Species | Mouse | ||||
| Protein Construction |
|
||||
| Purity |
> 95%?as?determined?by?Bis?Tris?PAGE? > 95%?as?determined?by?HPLC |
||||
| Endotoxin Level | Less than 1 EU per μg by the LAL method. | ||||
| Biological Activity | FcRn, His, Mouse captured on CM5 Chip via anti?his antibody can bind Trastuzumab in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 32.1 kDa (FCGRT)&11.7 kDa (B2M) | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of FcRn, His, Mouse is greater than 95% as determined by SEC-HPLC. ?
FcRn, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. ?
FcRn, His, Mouse on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200) (QC Test). ?
FcRn, His, Mouse on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 0.18 ?M as determined in SPR assay (Biacore T200). ?
FcRn, His, Mouse, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). ?
FcRn, His, Mouse, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). ?
| Target Background | The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
| Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
