| 产物编号 | 名称 | 数量 | 价格 | 操作 |
|---|---|---|---|---|
|
A00186
|
THE? His Tag Antibody, mAb, Mouse |
|
￥2500.00 100 μg |
|
基于以上的特性，THE™ Anti-His mAb在Western blot，Dot blot，ELISA，免疫荧光染色，以及流式细胞技术等方面有广泛应用，并能取得良好的实验效果。
| 产物说明 | 规格 | 溶解 | 存储条件 |
|---|---|---|---|
| 抗His-tag的单克隆抗体可以用于鉴定、检测和纯化细菌、昆虫细胞、哺乳动物细胞等表达系统表达的His融合蛋白。本公司以6xHis作为免疫原，获得了高亲和性和高特异性的单抗细胞株，以此制备小鼠腹水并通过protein G亲和纯化得到了THE™ Anti-His mAb（IgG1亚型）。 | 100μg冻干粉（缓冲液PBS pH7.4，含0.02%叠氮化钠）。 | 用100μl去离子水溶解冻干物THE™ His mAb（100μg）终浓度为1mg/ml。 |
|
应用
由于影响合适工作浓度的条件包括：二抗浓度，监测方法的灵敏度，温度及孵育时间等，所以实验人员进行具体实验时需要自行测定本产物的合适使用浓度。
使用本产物时的推荐浓度为：
注：在运输和储存过程中，可能会有少量的抗体粘附在包装瓶口处。使用前需使用台式离心机轻微离心以让粘附的产物离心到包装瓶的底部。
Figure 1：Sensitivity comparison of 6xHis-tag Fusion Protein by Western blot using His Antibody，mAb，Mouse from GenScript（A00186，0.1 μg/ml）and Qiagen（34698，0.1 μg/ml）.
Figure 2：Western blot analysis of N-terminal and C-terminal 6xHis-tag Fusion Protein using THE™ His Antibody，mAb，Mouse（A00186，1 μg/ml）.
Figure 3：Sensitivity comparison of 5xHis-GST in Whole Cell Lysates by Dot blot using His Antibody，mAb，Mouse from GenScript（A: A00186，1 μg/ml）and Qiagen（B: 34698，1 μg/ml and C:34660，1 μg/ml）.
Figure 4：Sensitivity comparison of 4xHis-GST in Whole Cell Lysates by Western blot using His Antibody，mAb，Mouse from GenScript（A00186，1 μg/ml）and Qiagen（34698 ，1 μg/ml）.
Figure 5：Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THE™ His Antibody，mAb，Mouse（A00186）.
Figure 6：Consistency analysis of THE™ His Antibody，mAb，Mouse（GenScript，A00186，1 μg/ml）with His-tagged fusion protein by Western blot.
Figure 7: Immunocytochemistry/Immunofluorescence analysis of HEK293 cells（A）and HEK293 cells transfected with His tagged fusion gene（B）using THE™ His Antibody，mAb，Mouse（A00186）.
Figure 8: Flow cytometric analysis of HEK293 cells（A）and HEK293 cells transfected with His tagged fusion gene（B）using THE™ His Antibody，mAb，Mouse（A00186）.
| 产物编号 | 名称 | 数量 | 价格 | 操作 |
|---|---|---|---|---|
|
A00174
|
His-tag Antibody, pAb, Rabbit |
|
￥990.00 40 μg |
|
|
A00205
|
His-tag antibody [Biotin], pAb, Rabbit |
|
￥990.00 40 μg |
|
|
A00612
|
THE? His Tag Antibody [HRP], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A00613
|
THE? His Tag Antibody [Biotin], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01620
|
THE? His Tag Antibody [FITC], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01800
|
THE? His Tag Antibody [iFluor 488], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01801
|
THE? His Tag Antibody [iFluor 555], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01802
|
THE? His Tag Antibody [iFluor 647], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01857
|
MonoRab? Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit |
|
￥1980.00 40 μg |
|
